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    Home / Products / Visque Bio-Imaging Systems / Visque Bio-Imaging | SMART-LF System

    Visque Bio-Imaging | SMART-LF System

    A Compact Preclinical InVivo Fluorescent & Bioluminescent Imaging and Analysis System

    The VISQUE™ SMART-LF system is a compact but powerful  in vivo imaging system, providing high-quality bioluminescent and fluorescent imaging and analysis. Its fluorescent imaging capabilities offer a non-invasive way to study the pathophysiology of disease as well as responses to therapy. With bioluminescence imaging, researchers are also able to study ongoing biological processes, such as tumor growth. The SMART-LF is used in a wide range of applications, including cancer and cardiovascular studies, angiogenesis, stem cell biology, pharmacokinetics, and more. Serial assessment is possible for each live lab animal. The Visque SMART-LF system provides the maximum options in a compact system for cutting-edge researchers.

    Nude mouse image, six hours after injection of drug delivery material into the tail vein.

    Figure 1: Nude mouse image, six hours after injection of drug delivery material into the tail vein.


    SMART-LF’s
    in vivo Fluorescence and Bioluminescence Bio-Imaging

    Bio-imaging is an easy-to-use, fast but powerful in vivo technology for researchers to  non-invasively study molecular and biological processes of disease, track disease progression, and evaluate therapeutic effectiveness. Fluorescence and bioluminescence imaging each offer unique benefits for pre-clinical imaging of small laboratory animals. Fluorescence imaging is particularly useful for quantifying and monitoring the cell behavior of biological targets, and optimal for studying dynamic processes. With time-lapse imaging of live cells, researchers can acquire key information through observation over hours or days. The unique features of bioluminescence imaging provide precise quantification and specificity, allowing researchers to detect and monitor gene expression related to tumor growth, as well as to use for therapeutic monitoring. Bioluminescence imaging also is used to track bacteria, viruses, and other pathogens.

    READ MORE  to learn more about bioluminescence and fluorescence imaging and applications.


    Monitor disease progression and track disease phenomena over time

    SMART-LF’s fluorescence imaging is a fast, easy-to-use form of optical imaging that can provide two- and three-dimensional and functional information from a cellular scale up to several centimeters. It is relatively inexpensive and offers high throughput, as multiple animals can be imaged simultaneously and quickly. This makes it easier for researchers to image statistically significant population sizes. Visque SMART-LF fluorescence imaging allows researchers to track the abundance, location, and activity of specific molecules in vivo and over time.

    FIND OUT how bio-imaging can support your research — connect with our Senior Applications Engineer.


    Study ongoing biological processes in small laboratory animals, non-invasively

    SMART-LF’s bioluminescence imaging brings several advantages to pre-clinical research, allowing researchers to monitor living cells non-invasively within their natural environments. Visque’s advanced bioimaging technology provides for the measurement of tumor growth, visualization of developmental processes, and tracking of cell-cell interactions. Visque’s SMART-LF system offers high-sensitivity as well as high-quality bioimaging data in a fast, easy-to-use, and reasonably priced compact desktop system.


    High-sensitivity fluorescence imaging acquisition

    Several features of the Visque SMART-LF system contribute to its efficacy in fluorescent imaging. Visque’s scientific CMOS camera provides an optimized solution for high-end scientific applications, with a minimum pixel size of 26.5 µm. The camera’s high sensitivity and low noise capabilities allow researchers to precisely detect and quantify very weak signals in fluorescent imaging. The high-sensitivity sCMOS imaging sensor, with a high quantum efficiency (94% at 550nm) and low dark current, provides uniform image quality along with high-speed image acquisition (max. 37fps).


    Intelligent
    in vivo imaging viewer and kinetic analysis program

    The Visque SMART-LF system has superior imaging with fast and convenient analysis tools. The unique time-lapse imaging and analysis software was designed exclusively for the VISQUE InVivo Series. By using real-time image acquisition, more than ten analytic algorithms for pharmacokinetics and biodistribution research are supported.

    Image acquision and analysis

    Figure 2: Image acquision and analysis.

    Demonstrations are available for the Smart LF in our lab in Allentown or at your facility. 


    Special imaging and analysis features:

    • ONE-CLICK ANALYSIS: Automatic display of fluorescence level, ROI analysis, etc. 
    • EASY RE-ANALYSIS: Original images, analysis process, analyzed images, and ROI can be saved in a single file (*.cif)
    • FOUR DIFFERENT IMAGES IN ONE SCREEN: The display and analysis of four different images can be displayed and analyzed in a single screen
    • REPORT MODE: Raw image, ROI (Region of Interest), acquisition setup information, the Pseudocolor bar range, comments, and more

    Analyze four images on one screen.

    Figure 3: Analyze four images on one screen.


    Sophisticated design provides enhanced useability

    The Visque SMART system’s user-friendly product design makes it easy to manipulate small laboratory animals to acquire the fast-moving fluorescent imaging within their bodies. The lens can be easily adjusted in real-time with it’s motorized zooming lens (max. ƒ/1.8, 1x ~ 10x zoom (motorized zooming lens).

    The instrument is compact and lightweight, weighing only 22kg so it can be carried by hand. An exterior LED display makes it easy to see the Power, Connection, Door, and Acquisition statuses.

    Visque's user-friendly product design

    Figure 4: Visque’s user-friendly product design.


    Applications

    Bioluminescence and fluorescence imaging are easy-to-use, fast but powerful in vivo technologies for researchers to  non-invasively study molecular and biological processes of disease, track disease progression, or evaluate therapeutic effectiveness. Fluorescence and bioluminescence imaging each offer unique benefits for pre-clinical imaging of small laboratory animals. 

    Fluorescence imaging is particularly useful for quantifying and monitoring the cell behavior of biological targets, and optimal for studying dynamic processes. With time-lapse imaging of live cells, researchers can acquire key information through observation over hours or days. 

    The unique features of bioluminescence imaging provide precise quantification and specificity, allowing researchers to detect and monitor gene expression related to tumor growth, as well as to use for therapeutic monitoring. Bioluminescence imaging also is used to track bacteria, viruses, and other pathogens.

    Specifically, major uses of the Visque SMART-LF in vivo preclinical system include the following applications:

    • Pharmacology and toxicology
    • Oncology
    • Cardiovascular function
    • Photo-stability test of molecules
    • Cell therapy tests
    • Biodistribution of nanoparticles


    READ MORE  to learn more about bioluminescence and fluorescence imaging and applications.

    Pharmacokinetics study of exosomes labeled with NIR fluorescent dyes

    Figure 4: Pharmacokinetics study of exosomes labeled with NIR fluorescent dyes.


    Visque’s
    SMART and SMART-LF look similar but vary in imaging capability 

    A key difference between the two systems is that the SMART-LF system offers both bioluminescence and fluorescence imaging, while the SMART system provides fluorescence imaging only. However, there are some other differences that should be noted:

    • The camera sensor of the Smart-LF is a BSI sCMOS (Back Side Illuminated Scientific Complementary Metal–Oxide–Semiconductor) that provides very high sensitivity with quantum efficiency (QE) up to 94% at 550nm.
    • The SMART-LF resolution is 1824 x 1824 PPI compared to the SMART’s 1024 x 1024 PPI.
    • The SMART-LF camera has cooling ability to maintain the temperature of -50℃ from the ambient, but the SMART camera does not.
    • The maximum imaging speed (37fps) of the SMART-LF is higher than that (30fps) of the SMART.
    • The SMART-LF offers an optional heating stage to maintain the body temperature of experimental animals.

    There are many other aspects that remain very similar, including animal capacity (three mice), number of fluorescence filter slots (up to nine), and the CleVue software.


    FEATURES

    Longitudinal studies

    Data may be gathered for the same subjects repeatedly over a study period with minimal harm to the animal subjects, using simple anesthesia and no dissection.

    Compact size and ease-of-use

    The Visque SMART-LF system is a compact desktop instrument that weighs only 22kg and can be easily lifted and carried. The lens can be easily adjusted in real-time while the user-friendly product design makes it easy to manipulate small laboratory animals.

    Easy analysis and documentation

    One-click analysis provides automatic display of key values. Re-analysis and documentation are facilitated because objective data and images can be stored as a single file in a *.cif format. 

    Back Side Illuminated sCMOS camera 

    The camera sensor of the Smart-LF is a BSI sCMOS (Back Side Illuminated Scientific Complementary Metal–Oxide–Semiconductor) that provides very high sensitivity with quantum efficiency (QE) up to 94% at 550nm. Scientific CMOS, or sCMOS, is a breakthrough technology based on next-generation CMOS Image Sensor (CIS) design and fabrication techniques. 

    Uniform image quality with high-speed image acquisition

    The high-sensitivity imaging sensor provides rapid image acquisition (Max. 37fps). Quantum efficiency: Max. 94% @ 550 nm.

    Gas anesthesia adapter (optional)

    Anesthesia is used in many animal studies to immobilize animals while scanning or imaging. The SMART-LF system provides an optional gas anesthesia adapter to ensure continuous anesthetic gas flow and ensure complete animal immobility during imaging.

    Time-lapse imaging

    Various algorithms for Kinetics analysis are already embedded in SMART’s CleVueTM software. This is particularly useful for the following applications:  Cardiovascular analysis, study of lymphoid organ function, and pharmacokinetics.

    VIEW a detailed comparison of the VISQUE Bio-Imaging Systems.


    Micro Photonics Inc. provides instruments, laboratory services, training, and support from experts
    to help research scientists meet their most complex laboratory demands.

    Contact: Timothy Sledz, President / COO, Micro Photonics
    866-334-4MPI (4674).

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