A Versatile InVivo Fluorescent & Bioluminescent Imaging and Analysis System
The VISQUE™ ELITE System offers our highest in vivo imaging performance for both bioluminescent and fluorescent imaging and analysis. Its fluorescent imaging capabilities offer a non-invasive way to study the pathophysiology of disease as well as responses to therapy. With bioluminescence imaging, researchers are also able to study ongoing biological processes, such as tumor growth. The Visque ELITE high performance cooling camera, multi-wavelength fluorescence and bioluminescence Imaging, motorized full high definition lens, real-time imaging of up to five mice at a time, all provide faster imaging with enhanced ease-of-use. The ELITE addresses the widest range of research applications, including cancer, angiogenesis, liver function, neuroscience, inflammation research, stem cell biology, pharmacokinetics, cardiovascular, lymphoid organs, and more. For cutting-edge researchers, the ELITE provides the highest optical imaging performance options available in a desktop system.
Figure 1: The Visque ELITE desktop system.
Bio-imaging is an easy-to-use, fast but powerful in vivo technology for researchers to non-invasively study molecular and biological processes of disease, track disease progression, or evaluate therapeutic effectiveness. Fluorescence and bioluminescence imaging each offer unique benefits for pre-clinical imaging of small laboratory animals. Fluorescence imaging is particularly useful for quantifying and monitoring the cell behavior of biological targets, and optimal for studying dynamic processes. With time-lapse imaging of live cells, researchers can acquire key information through observation over hours or days. The unique features of bioluminescence imaging provide precise quantification and specificity, allowing researchers to detect and monitor gene expression related to tumor growth, as well as to use for therapeutic monitoring. Bioluminescence imaging also is used to track bacteria, viruses, and other pathogens.
READ MORE to learn more about bioluminescence and fluorescence imaging and applications.
The ELITE’s fluorescence imaging is a fast, easy-to-use form of optical imaging that can provide two-dimensional and functional information from a cellular scale to several centimeters. It is relatively inexpensive and offers high throughput, as multiple animals can be imaged simultaneously and quickly (up to five mice or one rat). This makes it easier for researchers to image statistically significant population sizes. Visque’s ELITE fluorescence imaging allows researchers to track the abundance, location, and activity of specific molecules in vivo and over time.
FIND OUT how bio-imaging can support your research — connect with our Senior Applications Engineer.
The ELITE’s bioluminescence imaging brings several advantages to pre-clinical research, allowing researchers to monitor living cells non-invasively within their natural environments. Visque’s advanced bio-imaging technology provides for the measurement of tumor growth, visualization of developmental processes, and tracking of cell-cell interactions. Visque’s ELITE system offers high-sensitivity as well as high-quality bio-imaging data in a fast, easy-to-use, and reasonably priced system for cutting-edge researchers.
Figure 2: Imaging of five mice simultaneously at 1.5x zoom (FOV:21cm x 16cm)
Unique among optical imaging systems for experimental animals, the Visque in vivo ELITE can shoot bioluminescence and fluorescence imaging from the green to near-infrared region, which corresponds to 500 to 870 nm. Real-time imaging allows researchers to shoot up to 24 pictures in a second. With this real-time imaging function, researchers can perform in vivo molecular function and pharmacokinetic analysis. This all contributes to the widest range of research applications, from cancer, vascular system, liver function, nervous system, immune-related diseases and stem cell research to cardiovascular, lymphatic and pharmacokinetic studies, all by using the exclusive real-time imaging.
ELITE is the world’s only in vivo imaging system offering both convenient real-time imaging and a precise experimental environment. This is achieved by a special design combining an inner door, a sliding stage, a preview feature for monitoring the interior of the imaging chamber in real time, and a foot switch that allows the researcher to start the image acquisition while handling the subject with both hands.
For examples of how to acquire and analyze real time images, watch the videos on the Vieworks website (www.vieworks.com).
High Performance Cooling Camera
The ELITE’s high performance is based on a new generation of cooled scientific CMOS detectors, which have a combination of thermoelectric cooling technology and radiating structure, cooling up to -20℃ below the ambient temperature (patent applied for). This has advantages over cooling systems with gas refrigerant, which are very failure prone, require frequent replacement of gas refrigerant, and have a high cost of maintenance. Adding to the high performance, the high-sensitivity sensor has a Pixel Pitch of 5.5 ㎛ and a Dynamic Range of 64 dB.
Multi-wavelength Fluorescence and Bioluminescence Imaging
Unique among optical imaging systems for experimental animals, the Visque in vivo ELITE can shoot bioluminescence and fluorescence imaging from the green to near-infrared region, which corresponds to 500 to 870 nm at a Frame Rate of 24 fps.
Motorized Full HD Lens
Precise control is achieved with the motorized lens, providing 1x – 6x zoom with nine steps, 21-step focus from 0 cm to 10 cm, and ten-step iris control from f/1/8 to closed. A varying field of view from 5 cm x 4 cm to 28 cm x 21 cm actually makes it possible to image more than five mice at one time (setting at 28 cm × 21 cm).
Real-time images for pharmacokinetics and cardiovascular system research
To perform studies such as pharmacokinetics, cardiovascular, and lymphatic system studies by measuring molecular events in vivo, researchers must acquire real-time images and then verify changes in position, velocity and quantity of the targeted molecules. The ELITE system can acquire up to 24 fps in real time.
Special Design for Real Time Imaging
When you acquire real time images of molecular movement, you need to begin the image acquisition immediately after injecting a probe or dye into the blood or lymphatic vessel of the animal subject to acquire accurate images. It is especially important for the fast moving molecules that an injection of a probe or dye is immediately followed by the image acquisition.
The InVivo Elite’s special design including double- layer doors allows you to begin the image acquisition immediately after providing stimulation or injecting a probe or dye into the animal subject. As described above, the InVivo Elite’s unique design (patent applied for) including inner door, sliding stage, preview feature (monitoring the interior of the imaging chamber in real time) and foot switch allows you to start the image acquisition while handling the subject with both hands. The world’s only in vivo imaging system with a unique design offers not only convenient real time imaging methods but also precise experimental environments.
Advanced Emission Filter & Motorized Filter Wheel
ELITE’s hard-coating emission filter minimizes autofluorescence and reflection, blocking up to level OD 7. Fluorescence imaging is the visualization of signal intensities emitted by a fluorescent dye after irradiating light at a specific wavelength (applicable to the dye) and then exciting the dye. To image positions and intensities of fluorescent signals accurately, only the signals emitted by the fluorescent dye must be imaged. The use of high quality filters is therefore essential to acquire an accurate fluorescent image without autofluorescence or reflection.
Up to nine emission filters with a motorized filter wheel
With ELITE’s motorized filter wheel, multiple filters can be mounted at the same time. One filter for imaging the shape of subjects and four filters for imaging green, red, far-red, and infrared fluorescence are provided. With these four default emission filters, most fluorescent dyes used in laboratories can be imaged, but five more customized filters can be added for additional options.
Unique Design for Real-time Imaging
The ELITE system offers the speed needed for real-time imaging, including the use of a foot switch that frees both hands for positioning the subjects, and also the double-door design that makes it possible to just use the inner door for near red infrared fluorescent imaging. Animal movement is minimized using a head holder integrated with an anesthetic gas adapter, and the sliding stage makes for exceptionally convenient operation. With the LCD Touch Screen, users can easily control external light, stage temperature, lens zoom, and imaging settings.
Convenient Control with Dedicated Software, CleVue™
CleVue software for in vivo signal detection and intelligent image analysis has been designed exclusively for the Visque systems. This dedicated software provides for advanced kinetic analysis, ROI analysis, and spectrum analysis. CleVue provides the color map and graph capabilities to verify changes in position, velocity, and quantity of targeted molecules at a glance. The software includes ten types of algorithms that allow for velocity measurements and quantifications of targeted molecules.
The Mean Transit Time (MTT) and Blood Flow Index (BFI) algorithms typically used in MRI for analyzing Cerebral Blood Flow (CBF) are included, as well as Visque’s exclusive algorithms (Trising, Imax, Fluangio, Dyangio, etc.) that have been achieved in cooperation with researchers in the Korea Advanced Institute of Science and Technology (KAIST) and Kyung Hee University. Several studies and research performed with these exclusive algorithms have already been cited in key Science Citation Index (SCI) journals. This ensures that the CleVue algorithms are very applicable to in vivo optical imaging.
LED (Irradiation Light Source)
The ELITE uses LEDs as an irradiation light source for fluorescence imaging. LEDs provide lower power consumption and more stable operation than lasers. With multiple two-watt high power LEDs, the ELITE produces greater light output than conventional laser-based light sources. Typically, LEDs emit light at one specific wavelength, however they also emit other light at shorter or longer wavelengths. The ELITE is designed to pass the LED light through the excitation filter and then irradiate the fluorescent dyes. This enables only the light at a specific wavelength required to excite the fluorescent dye to be irradiated accurately.
Data can be gathered for the same subjects repeatedly over a study period with minimal harm to the animal subjects, using simple anesthesia and no dissection.
Easy analysis and documentation
One-click analysis provides automatic display of key values. Re-analysis and documentation are facilitated because objective data and images can be stored as a single file in a *.cif format.
Advanced Scientific Cooling Camera
ELITE’s cooling camera is ready to use immediately after powering up. The camera provides fluorescence and bioluminescence imaging from visible to near infrared wavelengths (green to near infrared region) The exclusive design (patent applied for) for real-time fluorescence imaging shoots up to 24 fps. The imaging chamber of the ELITE is light-tight, so that no light penetrates from the outside. It provides the best bioluminescence imaging for low luminescent signals, which require long exposure time.
Uniform image quality with high-speed image acquisition
The high-sensitivity imaging sensor provides rapid image acquisition (Max. 30fps) Specifications: Quantum Efficiency: 72% at 595nm; Dynamic Range: 87dB; Dark Current: <10 e-/s/pix @ 30 OC.
Various algorithms for Kinetics analysis are already embedded in SMART’s CleVueTM software. This is particularly useful for the following applications: cardiovascular analysis, study of lymphoid organ function, and pharmacokinetics.
For examples of how to acquire and analyze real time images, watch the videos on the Vieworks website (www.vieworks.com).
VIEW a detailed comparison of the VISQUE Bio-Imaging Systems.
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