Applications of X-Ray Microscopy for Murine Femora Studies

Micro-CT imaging is highly effective for the assessment of trabecular and cortical bone morphology, allowing researchers to measure bone microarchitecture without relying on stereologic models. Researchers can non-destructively acquire the three-dimensional architecture of bone from any site within a small animal. The sample remains intact, allowing researchers to follow up with genetic and histological analysis. Because rodent models can mimic aspects of a biological process or disease found in humans, they are widely used in many types of biomedical research.

Micro-CT analysis of long bones in mice, illustrating a curved tibia bone. Skeletal Characterization of the Fgfr3 Mouse Model of Achondroplasia Using Micro-CT and MRI Volumetric Imaging. Shazeeb, Mohammed Salman, et al. Scientific Reports. 8. 10.1038/s41598-017-18801-0.

Skeletal Characterization of the Fgfr3 Mouse Model of Achondroplasia Using Micro-CT and MRI Volumetric Imaging

Micro-CT provides a deeper understanding of skeletal abnormalities that will help guide the development of new and effective therapeutic approaches for the treatment of achondroplasia. “Achondroplasia, the most common form of dwarfism, affects more than a quarter million people worldwide and remains an unmet medical need. Achondroplasia is caused by mutations in the fibroblast growth factor receptor 3 (FGFR3) gene which results in over-activation of the receptor, interfering with normal skeletal development leading to disproportional short stature. Multiple mouse models have been generated to study achondroplasia.”



Guidelines for assessment of bone microstructure in rodents using micro-computed tomography

It is difficult to interpret reported results and to compare findings across different studies without consistent reporting of parameters related to image acquisition and analysis, standardized terminology, and key outcome assessments. This article provides recommendations “…regarding (1) standardized terminology and units, (2) information to be included in describing the methods for a given experiment, and (3) a minimal set of outcome variables that should be reported.” The experimental design will be determined by the research objective, but these guidelines will help to provide accurate, consistent reporting of micro-CT bone morphometry and density measurements.



Murine femur micro-computed tomography and biomechanical datasets for an ovariectomy-induced osteoporosis model

“The development of new effective and safer therapies for osteoporosis, in addition to improved diagnostic and prevention strategies, represents a serious need in the scientific community.” Micro-CT has become a key tool in identifying osteoporosis in animal models by the assessment of bone microarchitecture, resistance, and bone strength. High resolution images acquired by micro-CT analysis accurately quantify and depict the 3D microarchitecture of both cortical and trabecular bone. Improved techniques to assess bone quality are essential for the development of better diagnostic and therapeutic strategies.



Human and mouse bones physiologically integrate in a humanized mouse model while maintaining species-specific ultrastructure

Humanized mouse models are studied to replicate human-like bone physiology. Human and mouse bone architectures differ in multiple scales, but how chimeric human-mouse bone physiologically interacts and structurally integrates is not well known. This study shows that “…mouse and human-like tissue types coexist and structurally integrate in a chimeric bone organ while maintaining species-specific ultrastructural differences.”



Related Articles:

X-ray Microscopic Examination of Murine Femora

Learn more about how micro-CT is used for bone research.

X-ray Microscopic Inspection of Poultry Bones

Micro-CT of Toolmarks in Bone Using the Bruker SkyScan 1173

Growing Bone in Space: Studying Spaceflight-induced Osteoporosis (OP) with Micro-CT

Micro-CT of a Wishbone




Related Products

Related Articles


For all 3D imaging experts, computer scientists, and A.I. engineers, if you haven’t heard, there

US Partner Form

    Coming Soon:

    Customer access to tips and instructional videos, method notes, tutorials, application notes, and other content to support your research.


    Service Engineer opening for Micro-CT Systems

    We are looking for a service engineer to join our team of experts in helping advance research by providing technical support and maintenance for micro-CT systems across the United States.

    Please submit resumes to:

    Micro Photonics Inc.
    1550 Pond Road, STE 110
    Allentown, PA 18104



    WVC Annual Conference

    Las Vegas, NV
    February 19-21
    Booth 1484

    Privacy Policy

    What information do we collect? We collect information from you when you register on our site, place an order, subscribe to our newsletter or fill out a form. When ordering or registering on our site, as appropriate, you may be asked to enter your: name, e-mail address, mailing address, phone number or credit card information. You may, however, visit our site anonymously.
    What do we use your information for? Any of the information we collect from you may be used in one of the following ways:

    ; To personalize your experience (your information helps us to better respond to your individual needs).

    ; To improve customer service (your information helps us to more effectively respond to your customer service requests and support needs).

    ; To process transactions. Your information, whether public or private, will not be sold, exchanged, transferred, or given to any other company for any reason whatsoever, without your consent, other than for the express purpose of delivering the purchased product or service requested.

    ; To send periodic emails .The email address you provide for order processing, will only be used to send you information and updates pertaining to your order.Note: If at any time you would like to unsubscribe from receiving future emails, we include detailed unsubscribe instructions at the bottom of each email.

    Terms and Conditions

    Terms of Service for

    Introduction. Welcome to This website is owned and operated by Micro Photonics Inc. By visiting our website and accessing the information, resources, services, products, and tools we provide, you understand and agree to accept and adhere to the following terms and conditions as stated in this policy (hereafter referred to as ‘User Agreement’), along with the terms and conditions as stated in our Privacy Policy (please refer to the Privacy Policy section below for more information).

    This agreement is in effect as of October 25, 2018.

    We reserve the right to change this User Agreement from time to time without notice. You acknowledge and agree that it is your responsibility to review this User Agreement periodically to familiarize yourself with any modifications. Your continued use of this site after such modifications will constitute acknowledgment and agreement of the modified terms and conditions.

    Responsible Use and Conduct. By visiting our website and accessing the information, resources, services, products, and tools we provide for you, either directly or indirectly (hereafter referred to as ‘Resources’), you agree to use these Resources only for the purposes intended as permitted by (a) the terms of this User Agreement, (b) the terms of any applicable confidentiality, non-disclosure, or other agreement between you and a third party, and (c) applicable laws, regulations and generally accepted online practices or guidelines.

    Call Now Button