Applications of Micro-CT to Study Seed Development

Microcomputer tomography (microCT) as a tool in Pinus tree breeding: pilot studies

Pinus breeding programs yield high numbers of seeds, but a non-destructive method to determine seed viability was needed. The Pinus species has a long reproductive cycle — up to 28 months — so determining when fertilization occurs is helpful when applying tissue culture methods. In this study, “the microCT data for extracted seeds of the three species identified some seeds with large voids, which suggest non-viability (low to zero germination potential), and that was confirmed by the germination test.”This study confirms the feasibility of using micro-CT scans to confirm fertilization and determine seed viability in Pinus tree breeding programs.

READ MORE

…………………………………………………………………………………………………………………

Micro-CT imaging of tomato seeds: Predictive potential of 3D morphometry on germination

The percentage of 3D free space is the most effective parameter to predict germination outcomes for tomato seeds. In this study, a germination test was carried out along with the morphometric characterization of tomato seed internal structure using micro-CT scanning. The goal was to accurately evaluate the predictive potential of internal seed 3D morphology for germination outcomes. Micro-CT proved to be an effective tool to investigate the relationship between tomato seed 3D morphology and seed physiology.

READ MORE

………………………………………………………………………………………………………………

X-ray microtomography in comparison to radiographic analysis of mechanically damaged maize seeds and its effect on seed germination

Mechanical damage to seeds may affect seed germination and reduce the health and vigor of seed production. This study utilizes micro-CT scanning to evaluate mechanical damage to maize seeds and the effects on seed germination. Micro-CT provided a precise mechanical damage characterization of maize seeds that was associated with seed germination and vigor. It was found that the increased percentage of damaged area in the coronal planes through the endosperm and endosperm + embryo was directly related to the short seedling length and lesser dry biomass. “This imaging technique represents an efficient non-invasive procedure for high-resolution seed inspection and is very promising in evaluating mechanical damage, injuries caused by insects, embryonic abnormalities and other morphological alterations in seeds caused by biotic or abiotic agents.”

READ MORE

………………………………………………………………………………………………………………

High-Throughput Phenotyping of Morphological Seed and Fruit Characteristics Using X-Ray Computed Tomography

In the past, seed and fruit phenotyping has been primarily accomplished by manual measurement or extraction of morphological properties from 2D images. These methods are low in throughput and don’t allow for the evaluation of 3D characteristics and the internal morphology of the seeds and fruit. Micro-CT scanning offers an easy way to non-destructively record the external and internal 3D structures of seeds and fruits. In this study, researchers introduced a “…high-throughput image acquisition approach by mounting a specially designed seed-fruit container onto the scanning bed. The corresponding 3D image analysis software, 3DPheno-Seed&Fruit, was created for automatic segmentation and rapid quantification of eight morphological phenotypes of internal and external compartments of seeds and fruits.” These methods may provide robust tools for phenotyping morphological seed and fruit traits that could benefit crop breeding and functional genomics.

READ MORE

……………………………………………………………………………………………………………….

Related Articles:

Micro-CT Imaging for a Strawberry Seed

Micro-CT of a Staghorn Sumac

Micro-CT of Fruits

Applications of Micro-CT

Micro Photonics Inc. provides instruments, laboratory services, training, and support from micro-CT experts
to help research scientists meet their most complex laboratory demands.

More information: FREE BUYERS’ GUIDE

Request: FREE EVALUATION SCAN to see how micro-CT works with your application.

Contact: Benjamin Ache, Product Manager, Bruker Micro-CTs P: 610-366-7103 ext 115.

Related Products

Related Articles

Coming Soon:

Customer access to tips and instructional videos, method notes, tutorials, application notes, and other content to support your research.

Employment

While we are not currently looking to fill a specific position at Micro Photonics, if helping others advance their research is something you are passionate about, then feel free to send us your resume so that we can keep it for further considerations when a position does become available.

Please submit resumes to: info@microphotonics.com

Micro Photonics Inc.
1550 Pond Road, STE 110
Allentown, PA 18104

Events

Due to Covid-19 restrictions, we will not be attending tradeshows in the near future. We are participating in webinars and virtual tradeshows and will post links to those as they are available.

Privacy Policy

What information do we collect? We collect information from you when you register on our site, place an order, subscribe to our newsletter or fill out a form. When ordering or registering on our site, as appropriate, you may be asked to enter your: name, e-mail address, mailing address, phone number or credit card information. You may, however, visit our site anonymously.
What do we use your information for? Any of the information we collect from you may be used in one of the following ways:

; To personalize your experience (your information helps us to better respond to your individual needs).

; To improve customer service (your information helps us to more effectively respond to your customer service requests and support needs).

; To process transactions. Your information, whether public or private, will not be sold, exchanged, transferred, or given to any other company for any reason whatsoever, without your consent, other than for the express purpose of delivering the purchased product or service requested.

; To send periodic emails .The email address you provide for order processing, will only be used to send you information and updates pertaining to your order.Note: If at any time you would like to unsubscribe from receiving future emails, we include detailed unsubscribe instructions at the bottom of each email.

Terms and Conditions

Terms of Service for www.microphotonics.com

Introduction. Welcome to www.microphotonics.com. This website is owned and operated by Micro Photonics Inc. By visiting our website and accessing the information, resources, services, products, and tools we provide, you understand and agree to accept and adhere to the following terms and conditions as stated in this policy (hereafter referred to as ‘User Agreement’), along with the terms and conditions as stated in our Privacy Policy (please refer to the Privacy Policy section below for more information).

This agreement is in effect as of October 25, 2018.

We reserve the right to change this User Agreement from time to time without notice. You acknowledge and agree that it is your responsibility to review this User Agreement periodically to familiarize yourself with any modifications. Your continued use of this site after such modifications will constitute acknowledgment and agreement of the modified terms and conditions.

Responsible Use and Conduct. By visiting our website and accessing the information, resources, services, products, and tools we provide for you, either directly or indirectly (hereafter referred to as ‘Resources’), you agree to use these Resources only for the purposes intended as permitted by (a) the terms of this User Agreement, (b) the terms of any applicable confidentiality, non-disclosure, or other agreement between you and a third party, and (c) applicable laws, regulations and generally accepted online practices or guidelines.

US Partner Form









    Call Now Button